Antinematodal Potential of Leaf Extracts of Adiantum Raddianum and Artemisia Absinthium with the Model Organism Caenorhabditis elegans

Abstract

Nematodal diseases are treated with a number of drugs. However, because of their toxicity and the resistance that worms developed, it is important that we have to produce highly effective drugs that are both safe and effective. The purpose of this research was to exploit the potential of nematocidal effects of Adiantum Raddianum (A. Raddianum) and Artemisia Absinthium (A. Absinthium) against Model Organism Caenorhabditis elegans. worldwide parasitic nematodes can cause significant morbidity and mortality in humans and livestock. selected medicinal plants, Adiantum Raddianum (A. Raddianum) and Artemisia Absinthium (A. Absinthium) Antinematodal potential at the cellular level has been studied for the preparation of the extract, distilled water, methanol, and n-hexane were used.  Antinematodal, anti-egg hatching, Fluorescence microscopy and Gene expressions of selected genes gst-4 and hsp-6 were used in RT-PCR. The extracts of A. Absinthium methanol (LD50 = 3.0 mg/mL) and A. Raddianum D. water (LD50 = 4.80mg/mL) potent Antinematodal effect. Strong anti-egg hatching activity at (1mg/mL). Adiantum Raddianum methanol (LD50 = 3.12mg/ml), Artemisia absinthium methanol (LD50= 3.00mg/ml) had a strong effect on by C. elegans egg laying activity, resulting in a 50% reduction of egg laying when exposed to plant compounds. The methanol extracts of A. Absinthium and A. Raddianum had a potent apoptotic effect on the intestines, muscles, uterus (eggs), and gonads of c. elegans. Nematicidal effect of plants due to phytochemicals. Both plant extracts tested in tables 1.10 and 1.11 showed statistically significant effects (p < 0.05, often much lower) on at both stress-related gene in C. elegans. Genes gst-4 and hsp-6 shows strong expression during quantitative RT-PCR after treated with plant compounds. Our research recommended that both plants could be used as alternative treatments to newly developed, broad-spectrum Antinematodal drugs.